TABLE 1.

Primers used for amplification and Pyrosequencing analysis of the RRDR of rpoB in M. tuberculosis

Primer name	Sequence (5′-3′)	Source
rpoB.PCR.SGS.Forw2	TTTCGATCACACCGCAGACGTTa	SGS ABb
rpoB.biotin.Rev2	Biotin-GGCACGCTCACGTGACAGAC	Interactivac
F1	GCGATCAAGGAGT	Life Technologiesd
F7	CAGCCAGCTGAGCCAATTCA	Life Technologies
F9	ACCAGAACAACCCGCTGTCG	Life Technologies
F13	TGACCCACAAGCGCCGACTG	Life Technologies

^aA 3-nucleotide 5′ poly(T) tail was added.

^bScandinavian Gene Synthesis AB, Köping, Sweden.

^cThermo Hybaid, Ulm, Germany.

^dInvitrogen, Paisley, Scotland.