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. 2006 Jun;44(6):2133–2140. doi: 10.1128/JCM.00237-06

FIG. 3.

FIG. 3.

Immunoreactivities of the biotin-labeled chimeric antigens. (A) IgM Rec-ELISA performance with the biotin-labeled GST-EC2 and GST-EC3 antigens with Toxoplasma IgM-positive (C+) or IgM-negative (C−) serum samples (diluted 1:100) by using different antigen concentrations. (B) IgM Rec-ELISA performance with chimeric antigens (concentration, 500 ng/ml) assayed with different dilutions of a Toxoplasma IgM-positive serum. (C) Chimeric antigens (concentration, 250 ng/ml) were stored at 4°C intervals and assayed over time by the IgM Rec-ELISA. The results are expressed as the ratio of the OD measured with the Toxoplasma IgM-positive and -negative sera (OD C+ and OD C−, respectively).