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. 2006 Jun;44(6):2133–2140. doi: 10.1128/JCM.00237-06

TABLE 1.

Oligonucleotide primers used for construction of the EC2 and EC3 antigens

T. gondii gene Primer name Primer sequence
MIC2 K551 5′-GGACTAGTCGGCTCCCCCAGGATGCC-3′
K553 5′-CATCCAGTCCTGCTACCGCCACCAGACCAGACGCCACATCCAGC-3′
MIC3 K552 5′-GTGGCGTCTGGTCTGGTGGCGGTAGCAGGACTGGATGTCATGCC-3′
K555 5′-TGACGACCGAGCTACCGCCACCAGAGTTATCGCATTTGCAGGATG-3′
SAG1 K554 5′-ATGCGATAACTCTGGTGGCGGTAGCTCGGTCGTCAATAATGTCGC-3′
K556 5′-CCGCGGCCGCTAGCCGATTTTGCTGACCCTG-3′
GRA3 K563 5′-GGACTAGTCGGCTGGCTGCCTTGGGAGGCCTTG-3′
K565 5′-GCCGCGGTAGCACTACCGCCACCAGACAAACCAGGGCGATCTGTG-3′
GRA7 K564 5′-GCCCTGGTTTGTCTGGTGGCGGTAGTGCTACCGCGGCCACCGCG-3′
K567 5′-CCGGTTCGTTACTACCGCCACCAGAGAAATGAACTTCTTCTTGTTC-3′
M2AP K566 5′-GAAGTTCATTTCTCTGGTGGCGGTAGTAACGAACCGGTGGCCCTAG-3′
K568 5′-CCGCGGCCGCAGATTCAGACTCAGACGGAC-3′