TABLE 3.
Immunoreactivities of chimeric antigens with IgM antibodies in sera from adults with acquired T. gondii infectiona
| Serum sample | IgG level (IU/ml) | Cutoffb
|
IgM Rec-ELISA cutoffc
|
||
|---|---|---|---|---|---|
| ELFA-Toxo-IgM | ETI-TOXOK-M | GST-EC2 | GST-EC3 | ||
| B1 | 28 | 5.37 | 2.74 | 2.950 | 1.948 |
| B2 | 255 | 3.86 | 1.49 | 0.546 | 0.498 |
| B3 | 78 | 3.01 | 1.38 | 0.471 | 0.867 |
| B4 | 1,358 | 2.28 | 1.17 | 0.464 | 0.453 |
| B5 | 178 | 2.31 | 1.27 | 0.598 | 0.406 |
| B6 | 155 | 2.00 | 0.97 | 0.993 | 0.720 |
| B7 | 109 | 3.20 | 1.76 | 0.794 | 0.642 |
| B8 | 99 | 3.16 | 1.78 | 0.572 | 0.389 |
| B9 | 103 | 2.28 | 1.34 | 1.056 | 1.222 |
| B10 | 85 | 2.22 | 1.44 | 0.930 | 0.704 |
| B11 | 70 | 1.01 | 0.79 | 0.416 | 0.376 |
| B12 | 26 | 1.34 | 0.93 | 0.392 | 0.461 |
| B13 | 36 | 1.22 | 0.86 | 0.532 | 0.499 |
| B14 | 156 | 0.99 | 0.58 | 0.534 | 0.833 |
| B15 | 204 | 0.93 | 0.90 | 0.810 | 0.710 |
| B16 | 133 | 1.13 | 0.85 | 0.465 | 0.322 |
| B17 | 183 | 1.14 | 0.82 | 0.325 | 0.327 |
| B18 | 242 | 0.90 | 0.71 | 0.497 | 0.500 |
| B19 | 80 | 1.00 | 0.79 | 0.444 | 0.706 |
| B20 | 258 | 1.40 | 0.88 | 2.678 | 0.484 |
| B21 | 278 | 1.69 | 1.06 | 0.703 | 0.509 |
| B22 | 246 | 1.25 | 0.76 | 1.094 | 0.780 |
| B23 | 59 | 1.23 | 0.71 | 0.495 | 1.499 |
| B24 | 38 | 0.78 | 0.87 | 0.584 | 0.455 |
| B25 | 130 | 0.76 | 0.92 | 0.562 | 0.545 |
| B26 | 262 | 0.84 | 0.65 | 0.649 | 0.551 |
| B27 | 168 | 0.96 | 0.85 | 1.439 | 0.938 |
| B28 | 126 | 0.78 | 0.80 | 2.475 | 1.160 |
| B29 | 197 | 1.38 | 0.61 | 0.544 | 0.358 |
| B30 | 127 | 0.86 | 0.52 | 0.847 | 0.531 |
| B31 | 72 | 1.28 | 0.93 | 1.756 | 0.891 |
| B32 | 130 | 0.77 | 0.71 | 0.505 | 0.381 |
| B33 | 439 | 1.00 | 0.66 | 0.834 | 0.464 |
| B34 | 83 | 0.66 | 1.32 | 1.162 | 0.989 |
| B35 | 178 | 0.89 | 0.87 | 0.694 | 0.487 |
| B36 | 560 | 0.86 | 0.69 | 0.817 | 0.628 |
| B37 | 223 | 0.96 | 0.73 | 0.531 | 0.819 |
| B38 | 242 | 0.98 | 0.41 | 0.379 | 0.318 |
| B39 | 118 | 1.16 | 0.84 | 0.420 | 0.380 |
| B40 | 232 | 1.39 | 1.01 | 0.490 | 0.467 |
| B41 | 213 | 1.03 | 1.05 | 0.750 | 0.822 |
| B42 | 243 | 1.06 | 0.97 | 0.534 | 0.502 |
| B43 | 154 | 0.75 | 0.73 | 0.455 | 0.337 |
| B44 | 35 | 1.90 | 1.51 | 0.383 | 1.008 |
| B45 | 667 | 0.85 | 1.01 | 0.366 | 0.285 |
| B46 | 275 | 0.95 | 0.99 | 0.411 | 0.544 |
| B47 | 157 | 1.93 | 1.36 | 0.382 | 0.464 |
| B48 | 1,037 | 1.08 | 0.51 | 0.385 | 0.301 |
| B49 | 92 | 1.31 | 0.68 | 0.537 | 0.427 |
| B50 | 255 | 0.69 | 0.69 | 0.354 | 0.801 |
a
Serum samples (samples B1 to B50) were analyzed by IgM Rec-ELISAs with individual GST-EC2 and GST-EC3 antigens or commercial assays with whole-cell Toxoplasma antigens (ELFA-IgM and ETI-TOXOK-M).
b
The cutoff values for the commercial ELFA-Toxo-IgM and ETI-TOXOK-M assays were 0.65 and 0.45, respectively, as indicated by the manufacturers. Boldface type, values greater than the cutoff value.
c
The cutoff values for the IgM Rec-ELISA with the GST-EC2 and GST-EC3 antigens, calculated with the IgM-negative sera (groups A and C; n = 40), were 0.34 and 0.38, respectively. Boldface type, values greater than the cutoff value.