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Nested PCR of E. histolytica/E. dispar. A 374-bp fragment of the 30-kDa surface antigen was amplified from DNA purified from stool samples. Lanes: 1, HM1:IMSS; 2, DNA ladder; 3, AS2IR; 4 through 8, stool samples with E. histolytica/E. dispar cysts; 9 and 10, stool samples with E. histolytica/E. dispar trophozoites.
