FIG. 2.

kDNA signatures of T. cruzi obtained by LSSP-PCR. Amplified DNA fragments of parasites isolated by blood cultures from patients with different clinical forms of Chagas' disease were resolved in 7.5% polyacrylamide gels and silver stained. A, indeterminate form; B, megaesophagus (ME) with degrees of dilation II, III, and IV; C, megacolon (MC) and megacolon associated with the cardiac form (MC + C); D, cardiac form with degrees of lesions CI, CII, and CIII; E, cardiac form associated with megaesophagus and megacolon (ME+MC+C); F, cardiac form associated with megaesophagus (ME+C).