TABLE 1.
Primers for sequencing of rifampin and isoniazid resistance-determining regions
| Primer (reference) | Sequence (5′-3′) | Ta (°C) | Concn (nM) | Target | Product length (bp) |
|---|---|---|---|---|---|
| katGwgF1 | GTC CTC TAT ACC GGA CTA CGC | 61 | 120 | katG whole gene | 2,899 |
| katGwgR1 | TCG CAC ATC CAG CAC ATT TC | ||||
| katGP5 | GGT CGA CAT TCG CGA GAC GTT | 68 | katG codon 315, sequencing primers | 518 | |
| katGP6 (21) | CGG TGG ATC AGC TTG TAC CAG | ||||
| TB92 | CCT CGC TGC CCA GAA AGG GA | 56 | 150 | inhA promoter region | 248 |
| TB93 (29) | ATC CCC CGG TTT CCT CCG GT | ||||
| AhpC1 | GCC TGG GTG TTC GTC ACT GGT | 56 | 150 | oxyR-ahpC intergenic region | 359 |
| AhpC2 (28) | CGC AAC GTC GAC TGG CTC ATA | ||||
| RPOBF | GGG AGC GGA TGA CCA CCC A | 65 | 125 | rpoB RRDR | 350 |
| RPOBR (14) | GCG GTA CGG CGT TTC GAT GAA C | ||||
| TB146-F | CTT CTC CGG GTC GAT GTC GTT G | 64 | 300 | rpoB N-terminal region | 365 |
| TB146-R (8) | CGC GCT TGT CGA CGT CAA ACT C | ||||
| RpoB-C3F | GAG TAC GTG CCC TCG TCT GA | 56 | 300 | rpoB cluster 3 region | 319 |
| RpoB-C3R | ACT TGC GCA TCC GGT AGG TA |