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Sucrose gradient of HA-tagged Kex2p. Spheroplasts lysates from the indicated strains were separated on sucrose density gradients (22–60%), and fractions were collected from the top. Kex2HA, calnexin (Cne1p, ER marker), Kre2p (Golgi marker), and CPY (vacuole marker) in each fraction were detected by Western blotting.
