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Complementation of Δpro phenotype is sequence specific. Halo tests were performed with control GLY39, KEX2HA, Δprokex2HA, and Δprokex2HA + preprokex2 strains (1–4, respectively) or the Δprokex2HA strain transformed with plasmids expressing pre[TR]kex2 (5), pre[TR]prokex2 (6), or proregions of human furin, rat PC7, human PC5, human PACE4, rat PC4, mouse PC1, and mouse PC2 (7–13, respectively) fused to pre[TR]kex2.
