TABLE 2.
VetTB STAT-PAK test responses in cattle infected with M. bovis by aerosol or intratonsillar inoculationa
| Route | Wk after inoculation | Responder rate (no. of positive/total no. inoculated) |
|---|---|---|
| Aerosolb | −1 | 0/9 |
| 4 | 8/9 | |
| 8.5 | 8/9 | |
| 17 | 8/9f | |
| Intratonsillarc | −1 | 0/8 |
| 7 | 0/8 | |
| 10 | 3/8f | |
| 16 | 0/8f | |
| 18 | 8/8f | |
| Intratracheald | 0 | 0/6 |
| 2 | 0/6 | |
| 5 | 5/6 | |
| 9 | 3/6 | |
| 13 | 4/6 | |
| 16 | 6/6f | |
| Intranasale | 0 | 0/2 |
| 3 | 2/2 | |
| 6 | 2/2 | |
| 12 | 2/2 |
a
The VetTB STAT-PAK test utilizes a unique cocktail of several recombinant antigens, including MPB83, ESAT-6, and CFP-10, optimized by evaluation of antigen recognition patterns using MAPIA.
b
Cattle received 105 CFU of M. bovis (either strain 95-1315 [n = 5] or HC2005T [n = 4]) by the aerosol route.
c
Cattle received 4 × 104 CFU of M. bovis strain 95-1315 by instillation directly into the tonsillar crypts.
d
Cattle received 4 × 104 CFU of M. bovis strain T/91/1378 via intratracheal administration.
e
Cattle received 106 CFU of M. bovis strain AF2122/97 via intranasal administration.
f
After skin test.