Detection of serum antibodies by ElephantTB STAT-PAK kit in elephant TB before, during, and after treatment. The RT was performed as described in Materials and Methods. Results were measured with an optical reader and expressed in reflectance units (peak area). Each panel shows kinetics of antibody responses found by the RT with a set of serum samples collected over time from one elephant, as indicated (K, I, C, and L). The time of diagnosis by positive culture is shown by arrows, and treatment duration is indicated for each elephant.