Skip to main content
. 2006 Jul;13(7):758–763. doi: 10.1128/CVI.00097-06

FIG. 2.

FIG. 2.

Results of a conventional PCR-based assay for FHBP grouping. Three primer pairs, specific for FHBP variants 1 and 2 (v.1,2), variants 2 and 3 (v.2,3), and 16S rRNA genes, were used to amplify cognate and noncognate templates. The positions of DNA size standards in base pairs are shown at the right. (A) Variant-specific detection of plasmid templates. Lane 1, no template control; 2, pET21b-FHBP (strain MC58, variant 1); 3, pET21b-FHBP (961-5945, variant 2); 4, pET21b-FHBP (M1239, variant 3). (B) Detection of genomic DNA templates. Lane 1, MC58 wild type (WT); 2, 961-5945 WT; 3, M1239 WT; 4, MC58 FHBP knockout (KO); 5, 961-5945 FHBP KO; 6, M1239 FHBP KO.