TABLE 2.
Effect of IgG/IgG-IC blocking of the MAb 3G8-CD16 interaction on NK cell activity against K562 cells
| IgG sourcea | Inhibition of 3G8 binding (%)b | Median LU10/106 NK cellsc | % of controld | No. of assayse |
|---|---|---|---|---|
| None | 6.68 | 100 | 7 | |
| IgG006/0, IgG016/0 | 34 | 5.72 | 96 | 4 |
| IgG016/47 | 15 | 4.67 | 97 | 4 |
| IgG006/47 or IgG006/52 | 96 | 8.15 | 117 | 5 |
Assays were done using 1 mg of each IgG/IgG-IC preparation and enriched NK cells.
In each experiment, percent inhibition was calculated from the percentage of CD16+ CD3− cells that were detected by FACScan after incubation of 1 mg of purified total IgG with an enriched NK cell population. Values shown represent mean percent inhibition relative to the percentages of CD16+ CD3− cells in the enriched populations.
There was no significant difference in LU10/106 NK cells when means for the various sources of purified IgG were compared with that for the control (Student's t test).
The 100% control represents the mean LU10/106 NK cells in individual assays for each enriched NK cell population that was not incubated with purified IgG before addition of K562 target cells.
Results obtained using effector cells from both uninfected and SIVmac239-infected macaques were combined to determine means for percent inhibition and median LUs.