TABLE 1.
Neutralization titer and percent inhibition of MAb binding in the epitope-blocking ELISA produced by rabbit antisera to reference RRV strains and other alphaviruses
| Rabbit antiserum (location and yr of strain isolation) | Neutralization titerb | % Inhibitiona of MAb:
|
||
|---|---|---|---|---|
| 3B2c | G8 | B10 | ||
| RRV strains | ||||
| T48 (North Queensland, 1959) | 160 | 56 | 46 | 67 |
| NB5092 (Eastern New South Wales, 1969) | 160 | 56.5 | 62 | 64 |
| Ch19575 (western Queensland, 1976) | 640 | 75.5 | 54 | 0 |
| K1503 (East Kimberly, WA, 1984) | 320 | 65 | 17 | 0 |
| WK20 (West Kimberley, WA, 1977) | 320 | 67 | 18 | 0 |
| SW876 (southwest WA, 1987) | 160 | 50 | 82 | 61 |
| SW2191 (southwest WA, 1988) | 80 | 76 | 42 | 0 |
| Other alphaviruses | ||||
| Getah virus | 480 | 6.5 | 11 | 0 |
| Sindbis virus | 40 | 0 | 48 | 0 |
| BFV | 640 | 0 | 59 | 0 |
| Semliki Forest virus | 640 | 4 | 70 | 0 |
| Chikungunya virus | 40 | 0 | 70 | 0 |
a
The percent inhibition of MAb binding was calculated as 100 − [OD (test)/OD (negative control) × 100].
b
Against homologous virus.
c
Boldface indicates that the RRV-specific MAb 3B2 produced the best results.