TABLE 3.
Isolation and detection of Vibrio cholerae from water, 64-μm size fraction plankton, and 20-μm size fraction plankton samples collected from the aquatic environment of Mathbariaa
| Site | Tech- nique | Result for sampleb
|
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 2004
|
2005
|
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| March
|
April
|
May
|
June
|
July
|
Aug.
|
Sept.
|
Oct.
|
Nov.
|
Dec.
|
Jan.
|
Feb.
|
March
|
April
|
May
|
||||||||||||||||||||||||||||||||
| W | 64 | 20 | W | 64 | 20 | W | 64 | 20 | W | 64 | 20 | W | 64 | 20 | W | 64 | 20 | W | 64 | 20 | W | 64 | 20 | W | 64 | 20 | W | 64 | 20 | W | 64 | 20 | W | 64 | 20 | W | 64 | 20 | W | 64 | 20 | W | 64 | 20 | ||
| 1 | Culturec | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | C | C | C | C | C | C | O1 | O1 | C | C | C | C | C | C | C | C | C | C | C | C | ||||||||||
| CBHd | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |||||||||||||||||||||||||||||||
| DFA | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | 39 | O1 | O1 | O1 | O1 | O1 | O1 | O1 | O1 | ||||||||||||||||||||||
| 2 | Culture | ND | ND | ND | ND | ND | ND | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | 39 | 39 | 39 | C | C | C | |||||||
| CBH | ND | ND | ND | ND | ND | ND | ND | ND | ND | |||||||||||||||||||||||||||||||||||||
| DFA | ND | ND | ND | ND | ND | ND | O1, 39 | O1, 39 | O1 | O1, 39 | 39 | O1 | 39 | O1, 39 | O1, 39 | 39 | O1 | O1 | O1 | O1 | O1 | O1 | O1 | 39 | O1 | O1, 39 | 39 | O1, 39 | O1 | |||||||||||||||||
| 3 | Culture | C | C | 39 | ND | ND | ND | C | C | C | C | C | C | C | C | C | C | C | C | 39 | C | 39 | C | C | C | C | C | C | C | C | C | C | 39, O1 | 39 | 39, O1 | C | C | C | ||||||||
| CBH | ND | ND | ND | ND | ND | ND | ND | ND | ND | 39 | ||||||||||||||||||||||||||||||||||||
| DFA | O1 | ND | ND | ND | O1 | O1 | O1, 39 | O1, 39 | 39 | 39 | O1, 39 | O1, 39 | O1, 39 | O1, 39 | O1 | O1, 39 | O1 | O1 | O1 | O1 | O1 | O1 | ||||||||||||||||||||||||
| 4 | Culture | C | C | C | ND | ND | ND | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | C | 39 | 39 | 39 | C | C | C | ||||||||
| CBH | ND | ND | ND | ND | ND | ND | ND | ND | ND | |||||||||||||||||||||||||||||||||||||
| DFA | 39 | ND | ND | ND | O1, 39 | O1 | 39 | 39 | 39 | 39 | O1, 39 | O1 | O1 | 39 | O1 | O1 | O1 | O1 | ||||||||||||||||||||||||||||
| 5 | Culture | C | C | C | ND | ND | ND | C | C | C | C | C | C | C | C | 39 | C | C | O1 | O1 | O1 | C | C | C | C | C | C | 39 | C | 39 | ||||||||||||||||
| CBH | ND | ND | ND | ND | ND | ND | ND | ND | ND | 39 | 39 | |||||||||||||||||||||||||||||||||||
| DFA | ND | ND | ND | 39 | O1, 39 | O1 | 39 | 39 | O1 | O1 | O1 | O1 | O1 | O1 | O1 | O1 | ||||||||||||||||||||||||||||||
| 6 | Culture | C | C | C | ND | ND | ND | C | C | C | C | C | C | C | C | 39 | O1 | O1 | O1 | C | 39 | 39 | 39 | |||||||||||||||||||||||
| CBH | ND | ND | ND | ND | ND | ND | ND | ND | ND | 39 | 39 | |||||||||||||||||||||||||||||||||||
| DFA | 39 | ND | ND | ND | O1, 39 | O1, 39 | O1, 39 | 39 | 39 | 39 | O1 | O1 | O1, 39 | 39 | O1 | O1 | O1 | O1 | O1 | |||||||||||||||||||||||||||
Samples were collected biweekly from six selected pond sites between March 2004, and May 2005.
W, water; 64, 64-μm size fraction plankton; 20, 20-μm size fraction plankton; C, culture positive for V. cholerae non-O1/non-O139; ND, not done; blank, negative; O1, V. cholerae serovar O1; 39, V. cholerae serovar O139.
Isolation of V. cholerae was done by selective enrichment followed by selective plating on TCBS and TTGA.
Colonies from LA were lifted onto nylon membranes for CBH, and toxigenic V. cholerae colonies from the master LA plate were picked (after regeneration of the colonies by reincubation at 37°C) based on positive CBH.