TABLE 2.
Effects of the presence of the autochthonous microflora and of oxygen on E. coli O157:H7 growth in rumen fluid obtained from sheep fed hay or hay plus wheat
| Dieta | No. of EHEC cells (log10 · ml−1) at 24 h of incubation − no. of EHEC cells at 0 h of incubation for indicated sampleb
|
|||
|---|---|---|---|---|
| Anaerobiosis
|
Aerobiosis
|
|||
| WOF | WF | WOF | WF | |
| H | 1.74 ± 0.41 | −(0.40 ± 0.43) | 3.65 ± 0.16 | 3.01 ± 0.02 |
| HW0 | 2.16 ± 0.11 | −(1.50 ± 0.56) | 4.00 ± 0.10 | 1.21 ± 0.29 |
| HW0 without substrate | ND | −(0.30 ± 0.1) | ND | ND |
a
Rumen samples were obtained from sheep fed hay or from sheep fed hay plus wheat before the morning feeding. For each diet, the effect of the presence of oxygen was significant (P < 0.0001), the effect of the presence of autochthonous flora was significant (P < 0.0001), and the effect of the interaction between oxygen and the flora was significant (P < 0.01).
b
Values are means ± standard deviations from three independent cultures. Growth is considered to have occurred if the difference is positive, and mortality was observed if the difference is negative. ND, not done.