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. 2006 Jun;72(6):3879–3886. doi: 10.1128/AEM.02266-05

FIG. 1.

FIG. 1.

Real-time amplification and detection of a 10-fold dilution series of E. coli strain 99507gfp ranging from nondiluted bacterial culture to 105-fold diluted bacterial culture added to soil prior to DNA extraction (equal to 5 × 106 CFU/DNA extraction down to 50 CFU/DNA extraction), including the optimal amount of E. coli strain 99507gfp (giving a CT value of 31). The fluorescence increase is plotted versus the cycle number of PCR.