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Transmission electron micrographs of EC1118 cells during the second fermentation of base wine. PMSF (1 mM) was added to the base wine 8 days after inoculation. After 8 h of incubation with the protease inhibitor, cells were removed and analyzed by TEM. The arrows indicate autophagic bodies in the vacuole. V, vacuole; N, nucleus; MIT, mitochondrion.
