TABLE 1.

Oligonucleotides used in this study

Target	Oligonucleotide name	Application	Sequence	Tma (°C)	G-C (%)	Reference(s)
choE	reqF	Q-PCR forward primer	5′-CGA CAA GCG CTC GAT GTG-3′	59	61	This study
	reqR	Q-PCR reverse primer	5′-TGC CGA AGC CCA TGA AGT-3′	59	56	This study
	reqP	TaqMan probe	5′-FAM-TGG CCG ACA AGA CCG ATC AGC C-TAMRAb-3′	69	64	This study
	COX-F	PCR forward primer	5′-GTC AAC AAC ATC GAC CAG GCG-3′	62.3	57.1	15
	COX-R	PCR reverse primer	5′-CGA GCC GTC CAC GAC GTA CAG-3′	64.7	66.7	15
vapA	RvapA114F	Q-PCR forward primer	5′-CAG CAG TGC GAT TCT CAA TAG TG-3′	59	48	This study
	RvapA188R	Q-PCR reverse primer	5′-GAA GTC GTC GAG CTG TCA TAG CT-3′	59	52	This study
	RvapA140P	TaqMan probe	5′-FAM-CAG AAC CGA CAA TGC CAC TGC CTG-TAMRA-3′	69	58	This study
	IP1	PCR forward primer	5′-AC TCT TCA CAA GAC GGT-3′	46	50	22, 30
	IP2	PCR reverse primer	5′-TAG GCG TTG TGC CAG CTA-3′	55.1	55.6	22, 30
vapB	H1	PCR forward primer	5′-TGA TGA AGG CTC TTC ATA A-3′	47.6	36.8	22
	H2	PCR reverse primer	5′-TTA TGC AAC CTC CCA GTT G-3′	53.2	47.4	22
IAC	IACP	TaqMan probe	5′-HEX-CGC CTG CAA GTC CTA AGA CGC CA-TAMRA-3′	68	61	24
hly	riacF	Forward primer IAC construction	5′-CGA CAA GCG CTC GAT GTG CAT GGC ACC ACC-3′	81	63	This study
	riacR	Reverse primer IAC construction	5′-CGA CAA GCG CTC GAT GTG ATC CGC GTG TTT-3′	78	57	This study

^aTheoretical melting temperature.

^bTAMRA, 6-carboxytetramethylrhodamine.