TABLE 2.
Specificity and sensitivity of vapA Q-PCRa
| Strain | Origin | Conventional PCR resultb
|
Q-PCR result for vapA | Concordancec | |
|---|---|---|---|---|---|
| vapA | vapB | ||||
| PAM 1126 | Horse | + | − | + | + |
| PAM 1286 | Iguana | − | − | − | + |
| PAM 1335 | Horse | + | − | + | + |
| PAM 1340 | Horse | + | − | + | + |
| PAM 1346 | Horse | + | − | + | + |
| PAM 1348 | Soil | − | − | − | + |
| PAM 1350 | Soil | + | − | + | + |
| PAM 1351 | Soil | + | − | + | + |
| PAM 1358 | Horse | + | − | + | + |
| PAM 1365 | Horse | + | − | + | + |
| PAM 1367 | Horse | + | − | + | + |
| PAM 1371 | Horse | + | − | + | + |
| PAM 1374 | Horse | + | − | + | + |
| PAM 1376 | Human | − | + | − | + |
| PAM 1387 | Unknown | − | − | − | + |
| PAM 1404 | Horse | + | − | + | + |
| PAM 1406 | Human | − | + | − | + |
| PAM 1408 | Horse | + | − | + | + |
| PAM 1410 | Horse | + | − | + | + |
| PAM 1413 | Human | − | + | − | + |
| PAM 1414 | Human | − | + | − | + |
| PAM 1415 | Human | − | − | − | + |
| PAM 1416 | Horse | + | − | + | + |
| PAM 1418 | Horse | + | − | + | + |
| PAM 1422 | Horse | + | − | + | + |
| PAM 1424 | Horse | + | − | + | + |
| PAM 1425 | Horse | + | − | + | + |
| PAM 1427 | Horse | + | − | + | + |
| PAM 1430 | Horse | + | − | + | + |
| PAM 1431 | Horse | + | − | + | + |
| PAM 1436 | Soil | + | − | + | + |
| PAM 1437 | Horse | + | − | + | + |
| PAM 1441 | Soil | − | − | − | + |
| PAM 1447 | Pig | − | + | − | + |
| PAM 1448 | Human | − | + | − | + |
| PAM 1453 | Horse | + | − | + | + |
| PAM 1463 | Human | − | − | − | + |
| PAM 1467 | Pig | − | + | − | + |
| PAM 1468 | Pig | − | − | − | + |
| PAM 1469 | Pig | − | + | − | + |
| PAM 1473 | Pig | − | + | − | + |
| PAM 1474 | Pig | − | + | − | + |
| PAM 1475 | Pig | − | + | − | + |
| PAM 1479 | Pig | − | + | − | + |
| PAM 1480 | Pig | − | + | − | + |
| PAM 1483 | Pig | − | − | − | + |
| PAM 1485 | Pig | − | − | − | + |
| PAM 1487 | Pig | − | − | − | + |
| PAM 1488 | Pig | − | − | − | + |
| PAM 1493 | Pig | − | + | − | + |
| PAM 1495 | Pig | − | + | − | + |
| PAM 1499 | Pig | − | − | − | + |
| PAM 1500 | Pig | − | + | − | + |
| PAM 1504 | Pig | − | − | − | + |
| PAM 1518 | Pig | − | − | − | + |
| PAM 1533 | Pig | − | − | − | + |
| PAM 1547 | Pig | − | − | − | + |
| PAM 1549 | Pig | − | − | − | + |
| PAM 1550 | Pig | − | − | − | + |
| PAM 1563 | Bovine | − | − | − | + |
a
Note that vapA+ and vapB+ scores are mutually exclusive, indicating that vapB is most likely an allelic variant of vapA. +, positive; −, negative.
c
Concordance between results of vapA conventional PCR and our vapA Q-PCR.