TABLE 3.
Distribution of VPAI in V. parahaemolyticus MTase gene-positive and V. parahaemolyticus MTase gene-negative strains of V. parahaemolyticus
| Strain | Serotype | V. parahaemolyticus MTase gene PCR product (683 bp) | Presence of 0.9-kb PCR product obtained with VPAI-screening primersa | Southern hybridization result forb:
|
||
|---|---|---|---|---|---|---|
| VP0380 (probe L) | V. parahaemolyticus MTase (probe M) | VP0403 (probe R) | ||||
| QM98284 | O3:K6 | +c | − | + | + | + |
| QM115996 | O3:K6 | + | − | + | + | + |
| AN-2416 | O3:K6 | + | − | + | + | + |
| AO-97 | O3:K6 | + | − | + | + | + |
| AP-24659 | O1:K56 | − | + | − | − | − |
| AP-19569 | O5:KUT | − | + | − | − | − |
| TW-271 | K8 | − | + | − | − | − |
| TW-288 | K6 | − | + | − | − | − |
| TW-421 | K29 | − | + | − | − | − |
| TW-491 | K15 | − | + | − | − | − |
| QM-113892 | Unknown | − | + | − | − | − |
| QM-113890 | Unknown | − | + | − | − | − |
| T8-9 | Unknown | − | + | − | − | − |
| T1-41 | Unknown | − | + | − | − | − |
| QM-112998 | Unknown | − | − | − | − | − |
| AP-33593 | O4:K55 | − | − | − | − | − |
| TW-414 | K29 | − | − | − | − | − |
| TW-435 | K60 | − | − | − | − | − |
| T7-25 | Unknown | − | − | − | − | − |
a
VPAI-screening primers (VP0379-F and VP0404-R) located outside VPAI (Fig. 1) are expected to amplify a 0.9-kb fragment from V. parahaemolyticus strains that lack VPAI.
b
See Fig. 1 for the locations of the three VPAI hybridization probes.
c
+, gene amplimer or Southern hybridization signal present; −, gene amplimer or Southern hybridization signal absent.