FIG. 5.

Time courses of phytate hydrolysis by the WT and mutant E228K PhyAs and the subsequent appearance and disappearance of the intermediate or end products from the hydrolysis. The values are expressed as the concentration relative to the possible complete (maximal) hydrolysis. The enzymes (2 U ml⁻¹) were incubated with 1% sodium phytate in 0.2 M glycine-HCl buffer, pH 3.5, for the designated times, and the hydrolytic metabolites were analyzed by HPLC. (A) Inositol hexaphosphate (IP6); (B) inositol pentaphosphate (IP5); (C) inositol tetraphosphate (IP4); (D) inositol triphosphate (IP3); (E) inositol diphosphate (IP2); (F) inositol monophosphate (IP1); (G) inorganic phosphate (Pi).