FIG. 1.

(A) Reverse transcriptase PCR with the F. pseudograminearum wild type (WT) and aurofusarin mutants PK2.1 and KHt62.1 and with primers designed for PKSs with an ER domain. (B) Reverse transcriptase PCR with the F. pseudograminearum wild type and mutants on three PKSs without an ER domain. The F. pseudograminearum strains were cultivated for 2 weeks on a medium described by Bell et al. (2). (C) Reverse transcriptase PCR with PKS4-specific primers for F. graminearum strain 1104-14, which produces high levels of ZON, after 4, 7, and 11 days of growth on polished rice. All PCR products are about 400 bp long. The PKS designations are based on those of Kroken et al. (23).