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Southern blot analysis of F. graminearum PH1, wild type (WT), and ΔPKS4 mutants. Genomic DNA was digested with BglII. (A) Analysis with probe made from a BglII/AsiSI digest of the hygB sequence from pAg1-H3-PKS4. (B) Analysis with probe made from a PCR product obtained with primers PKS4-del-1 and PKS4-del-2 from the deleted sequence of PKS4. (C) Analysis with probe made from a BglII/XhoI digest of the PKS4-1 sequence from pAg1-H3-PKS4. For the locations of probes, see Fig. 2.
