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Oxidation of cysteine in BYE broth. l-Cysteine, sterilized by filtration, was aseptically added to BYE broth. Samples were removed, and the concentration of cysteine was determined by the acid ninhydrin method as described in the text. The starting concentration of cysteine was estimated at 2.7 mM, which included the 2.3 mM supplement. No added iron, ▴; 0.025% ferric pyrophosphate, •; and 0.05% ferric pyrophosphate, ▪.
