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Growth kinetics of L. pneumophila wild-type and CYS-1 mutant strains. A. Growth of wild type with l-cysteine (•) or l-cystine (▪) and of CYS-1 with l-cysteine (○) or l-cystine (□) in BYE broth. B. Growth of wild type (▪) and CYS-1 mutant (•) in CDM medium supplemented with l-cystine. Turbidities were measured with a Spectronic 20 spectrometer in 300-ml sidearm flasks containing 100 ml of medium. Each time point represents the average of two determinations.
