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Energy-dependent uptake of l-[35S]cystine by the CYS-1 mutant and the WT and effect of CCCP. Cystine uptake was determined in PPB in the absence of energy sources (▪), in the presence of 1 mM l-serine and l-glutamate (•), and with energy sources in the presence of 10 μM CCCP (▴). WT L. pneumophila did not transport cystine (□).
