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. 2006 Jun;72(6):4001–4006. doi: 10.1128/AEM.02428-05

FIG. 3.

FIG. 3.

The tomato tQM gene product inhibits Bax-induced cell death in yeast. (A) The EGY48 strain was individually transformed with the empty vector pB42AD, pB42AD-tQM, pLexA-Bax, or pLexA-Bax plus pB42AD-tQM, and transformants were maintained on SD-galactose medium. A time course analysis of dead cell accumulation was employed during Bax-mediated yeast lethality. Evans blue staining was performed at 0, 4, 7, 12, and 24 h after culturing on SD-galactose medium. Dead cells were scored, and data shown are means ± standard errors of the mean of at least three independent experiments. (B) Transformants were treated with 50 μM DHR123 after 24 h of growth on SD-galactose medium and then observed with an epifluorescence microscope. The EGY48 strain was transformed with empty vector pB42AD (a), pB42AD-tQM (b), pLexA-Bax (c). or pLexA-Bax plus pB42AD-tQM (d).