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Linear regression analysis comparing qPCR result (y axis) and IFA (x axis) to quantify parasites after a 24-hour infection in m-ICcl2 (a) and Caco2A (b) cells. The parasite inoculum ranged from 103 to 106/ml, and these data are representative of two independent experiments performed in triplicate (panel a; n = 15) or in duplicate (panel b; n = 10). HPF, high-powered field.
