TABLE 1.
Proposed functions of deduced steffimycin gene cluster products
| ORF | No. of aaa | Proposed function in steffimycin biosynthesis | Closest similar protein, origin, (% identity/similarity), accession no. |
|---|---|---|---|
| orf1 | 483 | Putative IMP dehydrogenase SCL6.18c, S. coelicolor A3(2), (91/96), CAB76883 | |
| orf2 | 117 | Hypothetical protein SCL6.19c, S. coelicolor A3(2), (78/89), CAB76884 | |
| orf3 | 347 | Putative transcriptional regulator SCL6.20c, S. coelicolor A3(2), (96/98), CAB76885 | |
| orf4 | 228 | Ribulose-phosphate 3-epimerase SCL6.21c, S. coelicolor A3(2), (95/98), CAB76886 | |
| sfrA | 712 | Transmembrane efflux protein | Putative membrane protein similar to ActII-3 SAV6879, Streptomyces avermitilis MA-4680, (76/86), BAC74590 |
| stfS | 86 | Acyl carrier protein | Acyl carrier protein NcnC, Streptomyces arenae DSM40737, (64/80), AAD20269 |
| stfK | 406 | Chain length factor | Ketosynthase β Snoa2, Streptomyces nogalater ATCC 27451, (72/81), CAA12018 |
| stfP | 422 | Ketoacyl synthase | Ketosynthase, “Streptomyces steffisburgensis” JCM 4833T, (98/98), BAA92281 |
| stfOI | 111 | Monooxygenase | Oxygenase SnoaB, Streptomyces nogalater ATCC 27451, (47,65), CAA12015 |
| stfT | 274 | C-7 ketoreductase | Ketoreductase ChaL, Streptomyces chartreuses HKI-249, (46/72), CAH10174 |
| stfX | 146 | Fourth-ring cyclase | SnoO, Streptomyces nogalater ATCC 27451, (52/62), AAF01807 |
| stfY | 259 | Second- and third-ring cyclase | Putative polyketide cyclase SnoaM, Streptomyces nogalater ATCC 27451, (74/83), AAF01818 |
| stfRI | 290 | SARP family regulator | Putative SARP family pathway-specific regulatory protein ORF71, Streptomyces rochei 7434AN4, (55/73), BAC76529 |
| stfRII | 196 | Putative transcriptional regulator | Putative two-component response regulator Gra-orf10, Streptomyces violaceoruber Tu22, (49/69), CAA09631 |
| stfQ | 309 | Aromatase | Putative aromatase SAV2383, Streptomyces avermitilis MA-4680, (51/63), BAC70094 |
| stfMI | 225 | O-Methyltransferase | Putative O-methyltransferase EncK, Streptomyces maritimus, (40/59), AAF81726 |
| stfC | 187 | Unknown | SWIM Zn finger Franean1DRAFT_6984, Frankia sp. strain EAN1pec, (56/70), ZP_00567311 |
| stfD | 618 | Unknown | SNF2-related domain:helicase, C-terminal Franean1DRAFT_6983, Frankia sp. strain EAN1pec, (72/81), ZP_00567310 |
| sfrB | 502 | Transmembrane efflux protein | Putative transmembrane efflux protein SCE22.16c, Streptomyces coelicolor A3(2), (41/61), CAB90983 |
| stfRIII | 116 | Putative transcriptional regulator | Hypothetical DNA-binding protein YcgE, Bacillus licheniformis ATCC 14580, (31/59), AAU23698 |
| stfPI | 402 | Monooxygenase | Cytochrome P450 Franean1DRAFT_4258, Frankia sp. strain EAN1pec, (46/59), ZP_00570217 |
| stfE | 526 | Dehydrogenase | BusJ, Saccharopolyspora pogona NRRL 30141, (55/68), AAY88927 |
| stfOII | 387 | Monooxygenase | Possible dioxygenase Mb3186c, Mycobacterium bovis AF2122/97, (51/64), NP_856831 |
| stfMII | 391 | O-Methyltransferase | Putative O-methylase SnogY, Streptomyces nogalater ATCC 27451, (54/68), CAA12021 |
| stfF | 113 | Unknown | 5-Chloromuconolactone dehalogenase ClcF, Rhodococcus opacus 1CP, (38/66), CAD28145 |
| stfG | 420 | Glycosyltransferase | Putative glycosyltransferase SnogE, Streptomyces nogalater ATCC 27451, (62/74), AAF01809 |
| stfPII | 366 | Cytochrome P450-like | Protein similar to DnmQ AknT, Streptomyces galilaeus ATCC 31615, (36/47), AAF73456 |
| stfMIII | 392 | O-Methyltransferase | Methyltransferase MycE, Micromonospora griseorubida, (42/58), BAC57026 |
| orf29 | 1,404 | Hypothetical protein SAV4434, S. avermitilis MA-4680, (37/49), NP_825611 | |
| orf30 | 374 | Hypothetical protein FG11565.1, Gibberella zeae PH-1, (41/57), EAA78433 | |
| orf31 | 344 | Trypsin-like serine proteases Npun02003978, Nostoc punctiforme PCC 73102, (35/51), ZP_00110193 | |
| orf32 | 415 | Uncharacterized protein conserved in bacteria PphaDRAFT_1502, Pelodictyon phaeoclathratiforme BU-1, (41/57), ZP_00590365 | |
| orf33 | 475 | Conserved hypothetical Sun-family protein SCL6.29c, S. coelicolor A3(2), (90/95), CAB76894 | |
| orf34 | 310 | Methionyl-tRNA formyltransferase SCL6.30c, S. coelicolor A3(2), (89/95), CAB76895 | |
| orf35 | 183 | Hypothetical protein SAV6876, S. avermitilis MA-4680, (88/91), BAC74587 | |
| orf36 | 422b | Putative primosomal protein N′ SCL6.32c, S. coelicolor A3(2), (86/90), CAB76897 |
a
Number of amino acids of the deduced protein.
b
Incomplete.