TABLE 2.

Oligonucleotide primers used to make deletion mutants

Gene deletion	Strain (WAM)	Primer
degP	3155	Fwd, ACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACTGTGTAGGCTGGAGCTGCTTC
		Rev, GGAGAACCCCTTCCCGTTTTCAGGAAGGGGTTGAGGGAAACATATGAATATCCTCCTTAG
skp	3170	Fwd, AACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATGTGTAGGCTGGAGCTGCTTC
		Rev, ACTGCTGCGCTAAATCAGCCAGTCGAATTGAAGGCATTACCATATGAATATCCTCCTTAG
mdoG	3172	Fwd, ATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGATGTGTAGGCTGGAGCTGCTTC
		Rev, TCAACGTCTGATCGGCATTCACCAGCGCAGCACGCATTTCCATATGAATATCCTCCTTAG
surA	3179	Fwd, ATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTGTGTAGGCTGGAGCTGCTTC
		Rev, CCACGTAATCCGCAGTGCGGTTAATTGAAATGGAAAAAGTCATATGAATATCCTCCTTAG
cutC	3380	Fwd, GGTACGAGCAAGCATCATATTGGGCGACATGATGCAACGGTGTGTAGGCTGGAGCTGCTTC
		Rev, ACTGGTGACGGGCGTTCTCGATGTTGACGGGAATGTCGATCATATGAATATCCTCCTTAG
dsbC	3379	Fwd, ACTTCTCGGCGACGAAGTTGTATCTGTTGTTTCACGCGAATGTGTAGGCTGGAGCTGCTTC
		Rev, TATGTTATTTACCTTGTTGGCAGCGTTTTCAGGCTTTGTTCATATGAATATCCTCCTTAG
yggN	3384	Fwd, CGGCAATGACCGCTCACGCCGACTACCAGTGCAGCGTCACTGTGTAGGCTGGAGCTGCTTC
		Rev, GAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGCATATGAATATCCTCCTTAG
ytfJ	3394	Fwd, TGCCGATGATGGCTTCCGCACATCAGTTCGAAACCGGTCATGTGTAGGCTGGAGCTGCTTC
		Rev, TGCACCTCTTCCTGAGTAAGTGCCCCGTCTTTGGCCCATCATATGAATATCCTCCTTAG
ygiM	3395	Fwd, TAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATTGTGTAGGCTGGAGCTGCTTC
		Rev, GGTGCGGCAGTACCAGACCGAGCAGCAAGCCAAGCCCCAGCATATGAATATCCTCCTTAG
yraP	3368	Fwd, AACACGCTTTTCCCTCACCAGGATGTTTAAGGAGAATACTGTGTAGGCTGGAGCTGCTTC
		Rev, ATAAGACGCTGCAAGCGTCGCATCAGGCATTACAAGGGCCATATGAATATCCTCCTTAG
degQ	3144	Fwd, AATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATATGTGTAGGCTGGAGCTGCTTC
		Rev, ACCGGACATCACACGTCAGCCTGATGCCCGGTTTACGACACATATGAATATCCTCCTTAG
fkpA	3392	Fwd, GTTAACCCTGGGGTGAGATGCCCCGATCCTGGAGATATGGATGAGTAAAGGAGAAGAAC
		Rev, TAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAGTGCCATATGAATATCCTCCTTAG
yidQ	3393	Fwd, CGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTATGTGTAGGCTGGAGCTGCTTC
		Rev, TCAGTTATCAGGCATTCTGGCCGGTGGAATAACCGCATTCATATGAATATCCTCCTTAG