TABLE 3.
Escherichia coli strains constructed for this study
| Strain (WAM) | Parent (WAM) | Deleted locusa | Gene | Gene or construct description |
|---|---|---|---|---|
| 3340 | 2625 | b0161 | degP | (K-12), periplasmic chaperone, degradase |
| 3155 | 2267 | c0197 | degP | (CFT073), periplasmic chaperone, degradase |
| 3170 | 2267 | c0215 | skp | Outer membrane protein folding, chaperone |
| 3172 | 2267 | c1313 | mdoG | Periplasmic, glucan biosynthesis |
| 3179 | 2267 | c0066 | surA | Peptidyl-prolyl cis-trans isomerase |
| 3144 | 2267 | c3989 | degQ | Periplasmic serine protease |
| 3368 | 2267 | c3904 | yraP | Putative lipoprotein |
| 3379 | 2267 | c3473 | dsbC | Disulfide isomerase II |
| 3380 | 2267 | c2289 | cutC | Copper homeostasis |
| 3394 | 2267 | c3718 | ytfJ | Hypothetical protein |
| 3395 | 2267 | c3805 | ygiM | Hypothetical protein |
| 3398 | 2267 | c3544 | yggN | Hypothetical protein |
| 3392 | 2267 | c4121 | fkpA | Peptidyl-prolyl cis-trans isomerase |
| 3393 | 2267 | c4608 | yidQ | Hypothetical protein |
| 2880 | 2267 | c0457-9 | lacZYA | Lactose utilization |
| 3156 | 3155 | c0197 | degP | attTn7-degP+ complementation |
| 3157 | 3155 | c0197 | degP | attTn7-degPS210A complementation |
| 3341 | 3170 | c0215 | skp | attTn7-skp+ complementation |
| 3501 | 2267 | Wild-type CFT073 pACYC177-skp+ | ||
| 3502 | 3170 | c0215 | skp | CFT073 skp pACYC177-skp+ |
a
Locus designations are open reading frames in the MG1655 or CFT073 sequenced genomes.