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TEM studies with ruthenium red-stained cells (A and B) or cells treated with human serum and anti-C3 antibody-HRP conjugate (C and D). A and C, B-4500FO2 (wild type); B and D, TYCC645 (cps1Δ). The white arrow indicates the electron-dense layer, and the black arrow indicates the zone of exclusion as described in the text. CW, cell wall. Bar, 0.1 μm.
