FIG. 2.

Expression of truncated OVA protein (∼27 kDa) determined by Western blot analysis using a purified rabbit anti-ovalbumin antiserum. (A) Expression of OVA by L. monocytogenes ΔtrpS containing the expression plasmids indicated, grown to the late logarithmic phase in BHI medium. Lanes 1 to 5, proteins from culture supernatants; lanes 6 to 10, total cellular proteins. (B) Expression of OVA by COS-1 cells 3 days after transfection with the expression plasmids indicated. (C) Expression of OVA by Caco-2 cells 24 h after infection with 50 CFU/cell of L. monocytogenes ΔtrpS containing different expression plasmids. Proteins were obtained by immunoprecipitation prior to Western blot analysis. The arrowheads indicate bands representing truncated OVA protein. Lm, L. monocytogenes.