TABLE 1.

Sequences of oligonucleotide primers used to amplify MSP-1 and MSP-2 polymorphic regions of P. falciparum isolates from Gabon, 2000

Gene product	Primer	Sequence	Note
MSP-1	MSP1-C1	5′-AAGCTTTAGAAGATGCAGTATTGAC	Conserved
	MSP1-C2	5′-ATTCATTAATTTCTTCATATCCATC	Conserved
	K1a	5′-GAAATTACTACAAAAGGTGCAAGTG	K1 family specific
	K1b	5′-AGATGAAGTATTTGAACGAGGTAAAGTG	K1 family specific
	MAD20a	5′-AAATGAAGGAACAAGTGGAACAGCTGTTAC	MAD20 family specific
	MAD20b	5′-ATCTGAAGGATTTGTACGTCTTGAATTACC	MAD20 family specific
	RO33a	5′-TAAAGGATGGAGCAAATACTCAAGTTGTTG	RO33 family specific
	RO33b	5′-CATCTGAAGGATTTGCAGCACCTGGAGATC	RO33 family specific
MSP-2	MSP2-C1	5′-ATGAAGGTAATTAAAACATTGTCTATTATA	Conserved
	MSP2-C2	5′-CTTTGTTACCATCGGTACATTCTT	Conserved
	FC27a	5′-GCAAATGAAGGTTCTAATACTAATAG	FC27 family specific
	FC27b	5′-GCTTTGGGTCCTTCTTCAGTTGATTC	FC27 family specific
	3D7a	5′-AGAAGTATGGCAGAAAGTAAGCCTCCTACT	3D7 family specific
	3D7b	5′-GATTGTAATTCGGGGGATTCAGTTTGTTCG	3D7 family specific