Colocalization of O. tsutsugamushi with clathrin, α-adaptin, or caveolin 1. ECV304 cells were infected with O. tsutsugamushi for 30 min. The cells were subjected to anti-clathrin (A), anti-α-adaptin (B), or anti-caveolin 1 (C) antibodies with anti-O. tsutsugamushi antibody. Colocalization of each protein (green) with O. tsutsugamushi (red) was examined with a confocal microscope. Colocalization of each protein and O. tsutsugamushi produced yellow fluorescence. The ratios are the number of O. tsutsugamushi organisms that colocalized with clathrin (A), α-adaptin (B), or caveolin 1 (C) to the total number of infecting O. tsutsugamushi organisms. The colocalizations of O. tsutsugamushi with clathrin, α-adaptin, or caveolin 1 are indicated by arrows. Bars, 10 μm.