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. 2006 Jul;74(7):4246–4253. doi: 10.1128/IAI.01620-05

FIG. 3.

FIG. 3.

Colocalization of O. tsutsugamushi with EEA1 (A) and LAMP2 (B). Representative immunofluorescence with the confocal microscopic images of ECV304 cells infected with O. tsutsugamushi for the time periods indicated below. Cells were fixed, permeabilized, and double labeled with human serum against O. tsutsugamushi (goat anti-human rhodamine conjugate) and anti-EEA1 (Alexa Fluor 488) or LAMP2 (Alexa Fluor 488). (A) Merged images of EEA1 (green) and O. tsutsugamushi (red) at 30 min (left panels), 1 h (middle panels), and 2 h (right panels). (B) Merged images of LAMP2 (green) and O. tsutsugamushi (red) at 30 min, 1 h, and 2 h. The ratios are the number of O. tsutsugamushi organisms that colocalized with EEA1 (A) or LAMP2 (B) to the total number of infecting O. tsutsugamushi organisms. The colocalization of O. tsutsugamushi with EEA1 or LAMP2 is indicated by arrows. Bars, 10 μm.