Figure 3.

α5-mediated protection of cells from apoptosis triggered by various cytotoxic stimuli. Each cytotoxic reagent was added to culture medium at the beginning of the experiment. The vehicle, DMSO, was maintained at <0.1%. Data shown are representative of two independent experiments. (A) α5-mediated cell protection in RIE1 transfectants. Cells were treated with aspirin (upper panels) at 3.0 mM for 72 h, with staurosporine (middle panels) at 50 nM for 24 h, or with etoposide (lower panels) at 50 μM for 48 h before TUNEL analysis. α5-c10 is a clone expressing full-length α5; α5/1-c3 is a clone expressing a tailless α5. (B) α5-mediated protection in HT29 cells. Cells were treated with staurosporine for 48 h at 0.4 μM before TUNEL analysis. HT29 c28 is transfected with full-length α5.