Figure 6.

Expression pattern of the GS₁ gene members in the cotyledons of germinating seeds. A, RNA from cotyledons of germinating seeds were subjected to northern-blot analysis using the 3′-UTR of the five different GS₁ gene members. The hybridization profile with the 28S rRNA gene probe is shown as an indicator of RNA loads. B, Protein extracts (10 μg) from the cotyledons of seeds at different days following germination (as indicated) were subjected to SDS-PAGE followed by western-blot analysis using anti-GS antibodies. Root and nodule extracts were also included in the gel. GS α, β, and γ forms are indicated.