FIG. 2.

MaxiK function is required for the inflammatory response to bacterial virulence factors and to interleukin-1. Shown is TNF-α production from human blood macrophages (2 · 10⁵ cells per well in a 96-well dish) after 4 h of stimulation with either LPS (1 ng/ml), PG (1 μg/ml), LP (100 ng/ml), LTA (10 μg/ml), or IL-1 (10 ng/ml) in the absence or presence of the MaxiK blocker paxilline (20 μM) or the K⁺ channel blocker TEA (20 mM). The cytokine response to each stimulus in the absence of a channel blocker was set at 100%, and the corresponding data were normalized to these values. The absolute amounts of TNF-α in the supernatants of the stimulated cells are given above the rows. Data shown are means ± standard deviations from triplicate samples of one donor and are representative of three independent experiments with cells from different donors.