FIG. 3.

(A and B) Isolated lung leukocytes from untreated mice (Ctrl) or anti-CD4 Ab-treated (anti-CD4) C. neoformans-infected mice for (A and C) 2 and (B and D) 3 weeks were spun onto a glass slide and stained with Wright-Giemsa stain. Granulocytes were identified, and absolute numbers of granulocytes were calculated. *, P < 0.05 (n = 3 mice/group/time point). (C and D) Lung leukocytes were stained with CD45 and CD4 or CD8 or CD19 Abs. Cells were analyzed by flow cytometry. The percentages of CD4⁺, CD8⁺, or CD19⁺ cells were multiplied by absolute cell numbers to get total number of cells. The results are the mean of two independent experiments with n ≥ 6 mice/group. Mono, mononuclear cells; Neutro, neutrophils; Eos, eosinophils. *, P < 0.05; **, P < 0.005.