TABLE 1.
Leukocyte differentials for young versus mature mice
| Cell type | No. of leukocytes (106) at wka:
|
||
|---|---|---|---|
| 0 | 1 | 2 | |
| Total | |||
| Young | 15.6 ± 4.4 | 37.3 ± 11.0 | 120.8 ± 27.9 |
| Mature | 13.2 ± 1.1 | 38.7 ± 6.2 | 111 ± 18.0 |
| Mononuclear cells | |||
| Young | 14.7 ± 4.2 | 26.6 ± 6.5 | 82.7 ± 20.3 |
| Mature | 12.0 ± 1.5 | 27.8 ± 3.8 | 73.5 ± 12.6 |
| Neutrophils | |||
| Young | 0.59 ± 0.22 | 5.3 ± 1.9 | 13.9 ± 5.4 |
| Mature | 0.63 ± 0.04 | 5.9 ± 1.7 | 11.2 ± 3.5 |
| Eosinophils | |||
| Young | 0.18 ± 0.01 | 5.2 ± 3.6 | 23.8 ± 7.0 |
| Mature | 0.28 ± 0.11 | 4.8 ± 1.2 | 26.5 ± 9.7 |
Lung leukocytes were isolated from uninfected (week 0) or infected young and mature mice at weeks 1 and 2 postinfection. Cells were spun onto a glass slides, stained with Wright-Giemsa stain, and analyzed using a microscope. The absolute number of leukocyte subsets was calculated by multiplying the percentage by the total number of cells. Data are expressed as means ± standard error (n ≥ 4 mice/group/time point) and are representative of two independent experiments. Leukocyte numbers from young and mature mice were not statistically different from one another at any time point.