TABLE 3.
Cytokine production by lung leukocytes from young and mature mice
| Cytokine and mouse type | Cytokine production (pg/ml) at wka:
|
||
|---|---|---|---|
| 0 | 1 | 2 | |
| IL-4 | |||
| Young | 17.7 ± 8.0 | 63.5 ± 30.9* | 54.0 ± 45.4* |
| Mature | 9.5 ± 0.7 | 60.1 ± 18.1† | 58.0 ± 27.0 |
| IL-5 | |||
| Young | 2,859.5 ± 41.7 | 4,336.0 ± 866.6* | 3,478.6 ± 1,140.1 |
| Mature | 2,466.0 ± 1,142.6 | 3,054.7 ± 575.4 | 4,482.8 ± 1,519.3 |
| IL-10 | |||
| Young | 72.1 ± 29.6 | 298.2 ± 85.6** | 213.8 ± 36.0* |
| Mature | 56.1 ± 28.0 | 268.8 ± 131.8† | 207.6 ± 32.0† |
| IFN-γ | |||
| Young | 39.4 ± 7.8 | 326.0 ± 67.0** | 555.8 ± 121.0** |
| Mature | 50.1 ± 31.0 | 357.2 ± 57.8†† | 5,050.4 ± 90.5†† |
| TNF-α | |||
| Young | 826.3 ± 6.2 | 1,364.0 ± 274.8 | 1,198.0 ± 462.8 |
| Mature | 498.1 ± 75.1 | 1,319.2 ± 364.0 | 936.8 ± 129.5 |
a
Lung leukocytes were cultured for 24 h, and supernatants were collected. Cytokines IL-4, IL-5, IL-10, IFN-γ, and TNF-α were measured from uninfected (week 0) or infected mice by ELISAs. Data are expressed as means ± standard error (n ≥ 4 mice/group/time point). * and **, P < 0.05 and P < 0.005, respectively, when young infected mice are compared with young uninfected mice; † and ††, P < 0.05 and P < 0.005, respectively, when mature infected mice are compared with mature uninfected mice. Both groups of mice show similar induction of cytokines upon infection, and there were no significant differences between the two groups.