TABLE 2.
Effects of various growth conditions on feoB2 expression
| Conditions | feoB2 expressiona | 16S rRNA expressionb | feoB2/16S ratiob | Fold changec |
|---|---|---|---|---|
| Anaerobic incubation (−O2) | 14.4 ± 0.682 | 0.22 ± 0.011 | 65.45 | 1 |
| Exposed to air for 5 min | 16.7 ± 0.215 | 0.20 ± 0.024 | 83.50 | 1.28 |
| Exposed to air for 15 min | 57.8 ± 4.187 | 0.35 ± 0.011 | 165.14 | 2.52 |
| Exposed to air for 30 min | 100.4 ± 10.27 | 0.28 ± 0.068 | 358.57 | 5.48 |
| Anaerobic incubation with 200 μM H2O2 for 5 min | 21.1 ± 2.605 | 0.22 ± 0.030 | 95.91 | 1.47 |
| Anaerobic incubation with 200 μM H2O2 for 15 min | 22.8 ± 1.917 | 0.15 ± 0.01 | 152.00 | 2.32 |
| Anaerobic incubation with 5 mM EDTA for 15 min | 10.0 ± 0.557 | 0.13 ± 0.006 | 76.92 | 1.17 |
a
Expression rates were determined by real-time RT-PCR using fivefold serial dilutions of 100 ng of total RNA. Means and standard deviations of experiments performed in triplicate are shown.
b
Expression rates were normalized using 16S as an internal standard and are designated as feoB2/16S ratios.
c
The normalized feoB2 expression rate that was derived from anaerobically incubated cells (−O2) serves as the control and its fold change is designated as 1. Fold changes for feoB2 expression under different growth conditions were determined by dividing their corresponding normalized values by that of the control (−O2).