FIG. 4.
Cytokine production by OVA-specific CD4+ T cells. Five days after immunization, three spleens per group were pooled. Spleen cells were prepared and restimulated with OVA323-339. (A) Supernatants of cultures 3 days after restimulation of 3 × 106 T cells with 10 μg/ml of OVA peptide were tested for IFN-γ by ELISA. (B) Supernatants of cultures 3 days after stimulation of 1.5 × 106 T cells with 1 μg/ml of OVA peptide were tested for IL-4 by ELISA using triplicates. Means ± standard deviations of triplicate samples per group are shown. The results are representative of three independent experiments.