FIG. 6.
IFN-γ production by OVA-specific CD8+ T cells. Five days after immunization, three spleens per group were pooled. Spleen cells were prepared and stimulated with OVA257-264 (SIINFEKL) peptide (1 μg/ml). (A) Supernatants of cultures 3 days after restimulation were tested for the presence of IFN-γ by ELISA. (B) Flow cytometry analysis for intracellular IFN-γ was performed using anti-CD8-PE, anti-Vα2-FITC, and anti-IFN-γ-allophycocyanin antibody. Percentages of T cells positive for IFN-γ, OVA-specific TCR, and CD8 out of all CD8+ T cells are shown. Means ± standard deviations of triplicates per group are shown. The results are representative of three independent experiments.