TABLE 2.
Summary of PhoP-regulated Tn5-lacZ insertion mutants
| Mutant | Insertion sitea | Survival in macrophagesc | Annotatione |
|---|---|---|---|
| 28-16 | Unknown | + | Not applicable |
| 25-36 | 5′ to y0020 | + | YPO3722, methionine synthase |
| 91-8 | y0020 | ||
| 73-73 | y0455b | + | Hypothetical protein |
| 20-17 | y0513 | + | YPO0256, two-component sensor, spiR/ssrA |
| 32-70 | y0513 | ||
| 74-14 | y0513 | ||
| 110-13 | 3′ to y0564 | + | YPO0304, hypothetical protein |
| 51-62 | y0943b | + | YPO3247, putative adhesin |
| 88-65 | 3′ to y1820 | Defectived | YPO1660, Mg2+ transport ATPase protein C, mgtC |
| 62-41 | y1921 | Defective | YPO2418, mannosyltransferase family protein, pmrK/pqaB |
| 93-11 | y1921 | ||
| 105-55 | y1921 | ||
| 37-48 | y2124 | +d | YPO2292, putative lipoprotein |
| 95-70 | 3′ to 2124 | ||
| 21-29 | y2147 | Defective | YPO2174, nucleotide sugar dehydrogenase, ugd |
| 98-31 | y2147 | ||
| 100-53 | 3′ to y2563 | +d | YPO1744, hypothetical protein, pagP |
| 61-22 | y2608 | + | YPO1559, hypothetical protein |
| 31-05 | y2816 | +d | YPO1363, putative virulence factor, somA |
| 75-11 | y2816 | ||
| 41-48 | y3948 | + | YPO0164, putative membrane receptor protein |
a
Tranposon insertion sites are indicated by using locus tag nomenclature for ORFs from the KIM genomic sequence (accession no. NC_004088) (15).
b
Reading frames of lacZ and ORF are in inverse orientation.
c
The survival of bacteria carrying insertions in the genes was tested in J774A.1 cells using anti-Yersinia staining and immunofluorescence microscopy as described in Materials and Methods. A plus sign indicates that the survival phenotype was indistinguishable from that of the parental strain; defective indicates that survival was substantially reduced.
d
The macrophage survival phenotype was confirmed by insertion of the kan cassette into the corresponding ORF.