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. 2006 Jul;74(7):4064–4074. doi: 10.1128/IAI.00123-06

FIG. 3.

FIG. 3.

Schematic representation of the PCR assay for the detection of the jhp0870 and jhp0649-like genes in H. pylori strains (A) and analysis of their detection by PCR in ethidium bromide-stained 2,5% agarose gel (B). (A) The black arrows represent the forward F1-jhp0870/jhp0649, labeled F, and reverse F1-jhp0870/jhp0649, labeled R, conserved primers between the jhp0870 and jhp0649-like genes in H. pylori strains, respectively. These F and R primers hybridize both on jhp0649 and jhp0870-like genes. The three internal boxes in the jhp0870 scheme represent the 27-bp, 2-bp and 4-bp additional sequences present in jhp0870 but absent in jhp0649. (B) MW, molecular weight marker ΦX174 RF DNA/HaeIII fragments (72-1353 pb; Invitrogen).