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. 2006 Jul;74(7):4254–4265. doi: 10.1128/IAI.00386-05

TABLE 3.

Host gene expression modulated by T. gondii infectiona

Functional class Accession no.b Protein (gene) Fold change in transcription at h postinfectionc:
1 2 4 6 24 48 72
Transcription CAA58780 Transcription initiation factor TFIID (TAF11) 2.09 3.96 2.78 3.05 2.24 —d 1.91
AAH93797 DNA-directed RNA polymerase II, 16 kDa (POLR2D) — 2.34 — 2.02 — — —
AAH00889 DNA-directed RNA polymerase I, 16 kDa (POLR1D) 2.13 3.05 2.44 2.78 — — —
BAD06318 Signal transducer and activator of transcription 1 (STAT1) — 2.26 — — — — —
BAD89432 Signal transducer and activator of transcription 6 (STAT6) — 2.63 2.44 2.52 — — —
CAA65753 c-myc proto-oncogene product (MYC) — 2.44 2.29 2.07 — — —
AAH27924 Myc-binding factor Max (MAX) — 3.91 3.28 3.15 2.18 1.83 —
CAB40144 c-Fos protein (C-FOS)e — 2.65 2.43 2.65 — — —
AAH02844 Nuclear factor κB p100/p49 (NFKB2)e — — 1.89 1.82 — 1.65 —
Signal transduction AAH00471 Dual-specificity mitogen-activated protein kinase kinase 2 (MAP2K2) 2.22 4.05 2.35 4.43 2.17 — 1.86
AAM01193 Protein kinase C beta 1 (PKC) 2.04 3.53 2.69 3.14 2.11 — —
BAA06171 Tec protein-tyrosine kinase (TEC) — 2.28 — — — — −3.10
CAI23831 Proto-oncogene tyrosine-protein kinase LCK (LCK) — — — -2.33 — −2.11 −2.79
P80310 Calgranulin C (S100A12) — 3.15 2.34 2.55 2.03 — 1.90
AAH00846 Calcium- and integrin-binding protein 1 (CIB1) — 3.09 2.45 2.53 — — —
AAH25372 Calponin 3, acidic isoform (CNN3) — 2.73 — 2.22 — — —
AAS75333 Rho family small GTP-binding protein RhoG (RHOG) 2.32 4.13 3.21 3.27 2.37 2.05 —
AAV68380 GTP-binding protein SAR1b (SARA2) 2.22 3.68 2.73 3.10 2.14 1.95 1.94
CAB57359 Rab5 GDP/GTP exchange factor homologue (RABGEF1) — — — — — −2.89 −2.95
Immune response AAH07091 Interferon-inducible protein with tetratricopeptide repeats 1 (IFIT1) — 3.57 2.60 3.53 2.09 1.84 —
AAP35948 Interferon-inducible protein with tetratricopeptide repeats 4 (IFIT4) 2.15 3.80 2.66 2.76 2.26 1.91 —
AAP35538 Interferon-induced transmembrane protein 3 (IFITM3) 2.02 3.66 2.59 3.39 2.19 1.87 —
AAP30878 Proteasome activator 28 alpha subunit (PSME1) — 2.97 2.53 2.72 — — —
AAH21136 Interferon gamma-inducible protein 30 (IFI30) — 2.24 2.14 2.02 — — —
AAD38418 Immunoglobulin G heavy chain (IGHG) — 2.98 2.56 2.89 2.11 — —
AAC33488 Human cytokine receptor/Epstein-Barr virus-induced gene 3 (EBI3) 2.04 3.93 2.49 2.83 2.28 — 1.98
BAC98339 Interleukin-2 receptor gamma (IL2RG) 2.12 3.86 2.59 2.73 2.18 — —
AAH28221 Interleukin-8 receptor alpha (IL8RA) — 2.23 2.14 — — — —
AAQ84094 CXCL12 chemokine (CXCL12) — 2.56 2.12 2.07 — — —
AAZ32767 C-X-C chemokine receptor type 4 (CXCR4) 2.03 3.07 2.68 2.82 2.06 — —
AAH63122 Small inducible cytokine B9 precursor (CXCL9) — — — −2.80 — −2.23 −2.75
CAH72304 MHC class I-related protein (MR1) — — — — — — −2.27
BAB39859 MHC class II antigen, SLA-DM alpha chain (SLA-DMA) 2.04 3.73 2.65 2.89 2.11 — —
BAD92814 MHC class II antigen, HLA-DM beta chain (HLA-DMB) 2.33 3.22 2.67 3.08 2.13 1.82 1.84
BAD99115 MHC class II antigen, SLA-DQ alpha chain (SLA-DQA) 2.21 3.66 2.60 2.93 2.08 — —
BAD99117 MHC class II antigen, SLA-DQ beta 1 domain (SLA-DQB1) 2.28 4.12 3.11 3.37 2.36 1.94 1.80
BAD99121 MHC class II antigen, SLA-DR alpha chain (SLA-DRA) — — — — — — −2.00
AAR20889 Complement C1r component (C1R) 2.26 3.91 2.94 3.80 2.29 2.00 1.87
AAG40565 Complement component C3 (C3) — 2.29 2.11 2.21 — 2.58 2.44
AAR20891 Complement component C4 (C4) 2.25 4.26 2.48 2.86 2.29 1.83 1.85
Apoptosis AAC68653 BCL-2-interacting protein Beclin (BECN1)e — 2.41 2.17 2.19 — — —
BAA13115 Defender against cell death 1 (DAD1) — 3.66 2.43 2.59 2.23 — 1.82
AAC39171 Putative inhibitor of apoptosis (PIAP)e — 2.04 — — — — —
BC001602 CASP8- and FADD-like apoptosis regulator (CFLAR) 2.12 3.33 2.63 2.62 2.20 — —
AAC98530 p53-binding protein (TOPORS) 2.13 4.04 2.87 3.33 2.23 1.96 1.90
AAC69439 Inhibitor of p53-induced apoptosis-alpha (NME6) — 2.87 2.13 2.39 2.00 — —
AAH16040 Serine-threonine kinase 17b/DAP kinase-related apoptosis-inducing protein kinase 2 (STK17B) −2.61 — — −3.75 — −2.32 −3.48
P35638 Growth arrest- and DNA damage-inducible protein GADD153 (DDIT3) — — — — — −2.75 −2.87
CAA61857 Death-associated protein 5 (EIF4G2) — — — — — −2.16 —
BAD92726 Transformation/transcription domain-associated protein (TTRAP)e — — — — — −2.62 −2.40
Nutrient metabolism
    Lipid metabolism BAA22372 Squalene epoxidase (SQLE) — 2.70 2.20 2.33 — — —
AAH08466 Dolichol-phosphate mannosyltransferase (DPM1) — 2.46 2.24 2.00 — — —
AAH33841 Peroxisomal D3,D2-enoyl-coenzyme A isomerase, isoform 1 (PECI) — 2.98 2.75 2.80 — — —
BAA13965 Acyl-coenzyme A dehydrogenase (ACADL) 2.06 2.94 2.49 2.82 2.01 — —
P12026 Acyl-coenzyme A binding protein (DBI) 2.13 2.85 2.62 2.78 2.01 — —
AAB09475 Apolipoprotein AI regulatory protein1 (NR2F2) — 2.88 2.50 2.60 — — —
AAA30994 Apolipoprotein R (C4BPA) — 2.21 2.05 2.00 — — —
AAF33208 Glycolipid transfer protein (GLTP) — 2.64 2.15 2.14 — — —
    Carbohydrate metabolism AAH73741 Phosphoglycerate mutase 2 (PGAM2) — 2.50 — 2.17 — — —
AAQ15274 Pyruvate kinase, M1 isozyme (PKM1) — — — — — −2.27 −2.15
AAH93020 Isocitrate dehydrogenase 1, NADP+, cytosolic (IDH1) 2.06 3.62 2.40 2.75 — — 1.89
P16276 Aconitate hydratase, mitochondrial (ACO2) 2.04 2.94 2.16 2.47 — — —
AAA31017 Citrate synthase, mitochondrial (CS) — 2.76 2.31 — — — —
P33198 Isocitrate dehydrogenase (NADP), mitochondrial (IDH2) 2.03 2.69 2.58 2.26 — — —
AAC48610 Malate dehydrogenase, cytosolic (MDH1) — 2.34 2.05 2.06 — — —
AAB94003 Succinyl-coenzyme A synthetase (SUCLG1) — 2.09 — — — — —
    Oxidative phosphorylation AAD34190 ATP synthase F0 subunit 6 (ATP6) — 2.93 — — — −2.72 −2.51
AAF62364 Cytochrome b (CYTB) — — — — — −2.98 −2.70
AAQ06129 Cytochrome c oxidase subunit III (COX3) — — — — — −3.37 −2.70
AAQ06019 NADH dehydrogenase subunit 1 (ND1) — — — — — −2.35 —
AAR01789 NADH dehydrogenase subunit 4 (ND4) — — — — — −2.93 −2.96
    Nucleoside metabolism AAH26106 5′-Methylthioadenosine phosphorylase (MTAP) — 2.67 — — — — —
AAH07511 UMP synthase (UMPS) 2.32 3.73 2.77 3.22 2.48 2.00 2.04
AAA57475 Dihydropyrimidine dehydrogenase NADP+ (DPYD) — 3.07 2.34 2.42 — — —
AAH00293 Nucleoside-diphosphate kinase 1, isoform 2 (NME1) — 2.17 — 2.02 — — —
AAH02476 Nucleoside-diphosphate kinase 2, isoform 2 (NME2) — 2.09 — — — — —
Cell cycle AAC39263 DNase II (DNASE2) 2.36 4.15 2.66 3.40 2.22 — 1.99
AAH10022 CDC45-related protein (CDC45L) 2.29 4.06 2.54 2.98 2.22 — 1.92
AAH93642 Septin 7/CDC10 protein homologue (SEPT7) 2.36 3.43 2.61 2.90 2.10 — —
AAH11616 G1/S-specific Cyclin D3 (CCND3) 2.11 3.26 2.31 2.15 2.05 — —
AAH13919 Thymidylate synthetase (TYMS) — 2.86 2.47 2.64 2.00 — —
AAL91594 DNA polymerase beta (POLB) — 2.92 2.26 2.37 — −2.10 —
AAH14847 Origin recognition complex 4 (ORC4L) — 2.70 2.04 — — — —
Cell structure (cytoskeleton/ membranes) AAH15516 Proteoglycan 1, secretory granule (PRG1) 2.38 3.45 2.90 3.23 2.11 1.85 1.92
O02697 Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, gamma isoform (PIK3CG) — 3.26 2.89 2.86 — — —
AAH18034 Phosphatidylinositol-4-phosphate 5-kinase type II alpha (PIP5K2A) 2.08 3.05 2.61 2.50 2.08 — —
AAH36916 Phosphatidylinositol glycan, class O, isoform 2 (PIGO) — 2.88 — 2.19 — —
P50127 N-Acetyllactosaminide alpha-1,3-galactosyltransferase (GGTA1) 2.22 2.85 2.42 2.35 2.00 — —
AAH00590 Actin-related protein 2/3 complex subunit 2 (ARPC2) — 2.33 — — — — —
P60982 Destrin (actin-depolymerizing factor) (DSTN) — — — — — −3.10 −2.86
AAH10435 H1 histone family, member X (H1FX) — 2.62 2.26 2.25 — — —
Protein degradation AAH66336 26S proteasome-associated pad1 homolog (PSMD14) — — — — — −2.57 −2.18
CAI18623 Proteasome subunit beta type 8 (PSMB8) 2.03 3.02 2.49 2.87 — — —
AAH51289 Ubiquitin-conjugating enzyme E2I (UBE2I) 2.11 3.25 2.72 2.73 2.15 — —
AAH33349 Ubiquitin-conjugating enzyme E2D 2, isoform 1 (UBE2D2) — 3.28 2.42 2.66 2.05 — —
AAD28788 Ubiquitin fusion-degradation 1 protein (UFD1L) — 2.55 2.37 2.19 — — —
AAL32103 Ubiquitin ligase E3 alpha-I (UBR1) 2.16 3.43 2.41 2.78 — — 1.83
Protein synthesis AAG30114 Lysyl-tRNA synthetase (KARS) 2.04 3.07 2.72 3.07 2.11 1.97 —
AAD02220 Phenylalanyl-tRNA synthetase beta chain (FARSLB) — 3.04 2.49 2.60 2.08 — —
AAH01870 Eukaryotic translation initiation factor 2B, subunit 4 delta (EIF2B4) 2.20 3.94 2.74 3.07 2.24 — 1.91
AAH64908 Ribosomal protein S15 (RPS15) 2.01 3.08 2.43 2.65 2.00 — —
Protein trafficking AAH03382 Sorting nexin 2 (SNX2) −2.18 — — −3.29 — −2.29 −3.15
AAA51551 Alpha-2-macroglobulin precursor (A2M) — 3.25 2.32 2.37 — — —
AAK95565 Peptide/histidine transporter (SLC15A4) — 2.01 — — — — —
Cell growth and proliferation AAH96185 Proteinase 3/myeloblastin precursor (PRTN3) 2.16 3.36 2.41 2.50 — — —
AAP35490 Granulin (GRN) 2.17 3.06 2.39 2.64 — — —
AAH51321 Heparanase (HPSE) 2.05 2.90 2.25 2.31 2.00 — —
AAH00491 Proliferating cell nuclear antigen (PCNA)e — 2.11 — — — −2.05 —
Heat shock and stress response AAH16660 Heat shock 70-kDa protein 8, isoform 1 (HSPA8) — 2.55 2.54 2.43 — — —
AAH12807 Heat shock 90-kDa protein 1, beta (HSPCB) — 2.41 — — — — —
a

The T. gondii infection time course experiment was repeated twice, and hybridization of cDNA microarrays with fluorescently labeled cDNA probes was repeated twice per experiment. Modulated transcripts are classified according to functional groups. Only genes with ≥2-fold changes in transcription in T. gondii-infected cells versus uninfected control cells, relative to a GAPDH internal control, are shown.

b

From the NCBI protein database.

c

Values are average Cy3/Cy5 ratios from four replicate hybridization experiments (for P values determined by the t test, see the supplemental material at http://www.ag.unr.edu/ab/Downloads/sup2.xls for transcripts showing ≥2-fold changes in expression.

d

—, no significant change in gene expression.

e

Microarray data were confirmed by quantitative real-time RT-PCR.