TABLE 2.
Primers used in this study
| Primer name | Sequence (restriction enzyme engineered) | Fragment |
|---|---|---|
| TAF101 | 5′-GGAATTCGGCAAAGCGAGTGGGTGATTAG-3′(EcoRI) | asp24 upstream |
| TAF102 | 5′-CGGGATCCTGAGCAAGTGCGGGAATAGC-3′(BamHI) | asp24 upstream |
| TAF103 | 5′-CGGGATCCTGGGAATGGAGCGGCTTTAG-3′(BamHI) | asp24 downstream |
| TAF104 | 5′-GCTCTAGATTTGAACACTTGGCGATAGCG-3′(XbaI) | asp24 downstream |
| TAF300 | 5′-CGGGATCCCGCACGTCTTGAGCGATTGTGTAGG-3′(BamHI) | Kan cassette |
| TAF301 | 5′-CGGGATCCCGGGACAACAAGCCAGGGATGTAAC-3′(BamHI) | Kan cassette |
| TAF356 | 5′-CGGGATCCCTGGAGGAAAACAATCTGGG-3′(BamHI) | manBA upstream |
| TAF357 | 5′-AAGACGGCGCGCCCGAACCTGTATCTGCCTG-3′(AscI) | manBA upstream |
| TAF358 | 5′-GTTCGGGCGCGCCGTCTTAACCCAAAACCGCTTCGTA-3′(AscI) | manBA downstream |
| TAF359 | 5′-GCTCTAGAGGGTTTTCTGATCGATCTGGTAGC-3′(XbaI) | manBA downstream |
| TAF204 | 5′-GGCGCGCCACGTCTTGAGCGATTGTGTAGG-3′(AscI) | Kan cassette |
| TAF205 | 5′-GGCGCGCCGGACAACAAGCCAGGGATGTAAC-3′(AscI) | Kan cassette |